Sammanfattning

The objective of the study was to determine the antimicrobial susceptibility of 28 individual clinical isolates from horses, previously identified by phenotypic methods as Bacteroides spp., and to species identify these isolates using matrix-assisted-laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing. Rarely are Bacteroides spp. identified to species level, and treatment of infection with anaerobes is seldom guided by antimicrobial susceptibility testing. The gram-negative anaerobic rods were species identified using MALDI-TOF MS and 16S rRNA gene sequencing. Antimicrobial susceptibility was determined by nitrocefin hydrolysis and broth microdilution. The isolates were identified as Bacteroides fragilis, Bacteroides heparinolyticus, Bacteroides ovatus, Bacteroides pyogenes, Bacteroides thetaiotaomicron, Bacteroides sp., Parabacteroides distasonis, Porphyromonas sp., Prevotella sp., and Prevotella dentasini. All isolates had low minimum inhibitory concentrations (MICs) of metronidazole. beta-Lactamase production and high MICs of penicillin, cephalothin, ceftiofur, erythromycin, fusidic acid, and trimethoprim/sulfamethoxazole were recorded for B. fragilis, B. ovatus, and B. thetaiotaomicron. Parabacteroides distasonis did not produce beta-lactamase but were still resistant to penicillin and in addition to the aforementioned substances also had high MICs of tetracycline. Bacteroides heparinolyticus, B. pyogenes, and the isolates identified as Porphyromonas sp., Prevotella sp., and P. dentasini did not produce beta-lactamase and were generally more susceptible against the tested antimicrobial agents. It is concluded that antimicrobial susceptibility differs substantially between species of genus Bacteroides and it is difficult to species identify anaerobic gram-negative rods by conventional phenotypic methods. It is important both to species identify gram-negative anaerobic rods and perform antimicrobial susceptibility testing to ensure correct treatment. (C) 2016 Elsevier Inc. All rights reserved.

Nyckelord

Bacteroides spp.; Antibiotic resistance; Anaerobic bacteria; Horse; MALDI-TOF MS

Publicerad i

Journal of Equine Veterinary Science
2016, Volym: 45, sidor: 46-52
Utgivare: ELSEVIER SCIENCE INC

SLU författare

• Ovesen, Louise

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    

• Riihimäki, Miia

  • Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
    
Associerade SLU-program

AMR: Bakterier


Globala målen

SDG3 Säkerställa hälsosamma liv och främja välbefinnande för alla i alla åldrar


UKÄ forskningsämne

Klinisk vetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.jevs.2016.06.073

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/82971