Forskningsartikel2016Vetenskapligt granskadÖppen tillgång
METACASPASE9 modulates autophagy to confine cell death to the target cells during Arabidopsis vascular xylem differentiation
Escamez, Sacha; Andre, Domenique; Zhang, Bo; Bollhoner, Benjamin; Pesquet, Edouard; Tuominen, Hannele
Sammanfattning
We uncovered that the level of autophagy in plant cells undergoing programmed cell death determines the fate of the surrounding cells. Our approach consisted of using Arabidopsis thaliana cell cultures capable of differentiating into two different cell types: vascular tracheary elements (TEs) that undergo programmed cell death (PCD) and protoplast autolysis, and parenchymatic non-TEs that remain alive. The TE cell type displayed higher levels of autophagy when expression of the TE-specific METACASPASE9 (MC9) was reduced using RNAi (MC9-RNAi). Misregulation of autophagy in the MC9-RNAi TEs coincided with ectopic death of the non-TEs, implying the existence of an autophagy-dependent intercellular signalling from within the TEs towards the non-TEs. Viability of the non-TEs was restored when AUTOPHAGY2 (ATG2) was downregulated specifically in MC9-RNAi TEs, demonstrating the importance of autophagy in the spatial confinement of cell death. Our results suggest that other eukaryotic cells undergoing PCD might also need to tightly regulate their level of autophagy to avoid detrimental consequences for the surrounding cells.
Nyckelord
Arabidopsis thaliana; Autophagy; Intercellular signalling; Metacaspase; Programmed cell death; Tracheary element
Publicerad i
Biology Open
2016, Volym: 5, nummer: 2, sidor: 122-129
Utgivare: COMPANY OF BIOLOGISTS LTD
UKÄ forskningsämne
Cellbiologi
Publikationens identifierare
DOI: https://doi.org/10.1242/bio.015529
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/83013