Sammanfattning

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs) have been detected in humans and wildlife. Using in vitro models, we recently showed that OH-PBDEs disrupt oxidative phosphorylation (OXPHOS), an essential process in energy metabolism. The goal of the current study was to determine the in vivo effects of OH-PBDE reported in marine wildlife. To this end, we exposed zebrafish larvae to 17 OH-PBDEs from fertilisation to 6 days of age, and determined developmental toxicity as well as OXPHOS disruption potential with a newly developed assay of oxygen consumption in living embryos. We show here that all OH-PBDEs tested, both individually and as mixtures, resulted in a concentration-dependant delay in development in zebrafish embryos. The most potent substances were 6'-OH-BDE47 and 6-OH-BDE49 (No-Effect-Concentration: 0.1 and 0.05 mu M). The first 24 h of development were the most sensitive, resulting in significant and irreversible developmental delay. All substances increased oxygen consumption, an effect indicative of OXPHOS disruption. Our results suggest that the induced developmental delay may be caused by disruption of OXPHOS. Though further studies are needed, our findings suggest that the environmental concentrations of some OH-PBDEs found in Baltic Sea wildlife in the Baltic Sea may be of toxicological concern.

Nyckelord

zebrafish; OXPHOS disruption; hydroxylated PBDEs

Publicerad i

International Journal of Molecular Sciences
2017, Volym: 18, nummer: 5, artikelnummer: 970

SLU författare

• Dahlberg, Anna-Karin

  • Institutionen för vatten och miljö, Sveriges lantbruksuniversitet
    
  • Stockholms Universitet

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UKÄ forskningsämne

Utvecklingsbiologi
Annan kemi


Publikationens identifierare

DOI: https://doi.org/10.3390/ijms18050970

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/86181