Sammanfattning

We describe a method combining fluorescence-activated cell sorting (FACS) with one-step miniaturized isolation and accurate quantification of cytokinins (CKs) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to measure these phytohormones in specific cell types of Arabidopsis thaliana roots. The methodology provides information of unprecedented resolution about spatial distributions of CKs, and thus should facilitate attempts to elucidate regulatory networks involved in root developmental processes.

Nyckelord

Cytokinins; Fluorescence-activated cell sorting (FACS); Solid-phase extraction (SPE); Liquid chromatography-mass spectrometry (LC-MS); Roots; Arabidopsis; Protoplasts

Publicerad i

Methods in Molecular Biology
2017, Volym: 1497, nummer: 1497, sidor: 231-248
Titel: Plant Hormones
ISBN: 978-1-4939-6467-3, eISBN: 978-1-4939-6469-7
Utgivare: Humana Press

SLU författare

• Antoniadi, Ioanna

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

• Ljung, Karin

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Cellbiologi


Publikationens identifierare

DOI: https://doi.org/10.1007/978-1-4939-6469-7_1

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/87058