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Forskningsartikel2017Vetenskapligt granskadÖppen tillgång

1D NMR methods for determination of degree of cross-linking and BDDE substitution positions in HA hydrogels

Wende, Frida J.; Gohil, Suresh; Nord, Lars I.; Kenne, Anne Helander; Sandstrom, Corine

Sammanfattning

Hyaluronic acid polymers cross-linked with BDDE are today among the most used hydrogels for biomedical applications. The physical properties of the hydrogels depend, among other parameters, on the degree of cross-linking of HA. Another parameter likely to affect the physical properties is the substitution position of the linker on the HA functional groups. A NMR-based method for the determination of these parameters in hyaluronic acid hydrogels is presented. The method is based on the degradation of HA cross linked hydrogels by chondroitinase ABC followed by one-dimensional H-1 and C-13 NMR analysis. The necessary structural information to obtain both the degree of cross-linking and the substitution positions can be obtained from the same NMR sample and no chromatographic separation step is required prior to NMR analysis. (C) 2016 The Authors. Published by Elsevier Ltd.

Nyckelord

Hyaluronic acid; Hydrogels; NMR spectroscopy; Cross-linking ratio; Substitution position

Publicerad i

Carbohydrate Polymers
2017, Volym: 157, sidor: 1525-1530

      SLU författare

    • Wende, Frida

      • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
      • Gohil, Suresh

        • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet
        • Sandström, Corine

          • Institutionen för kemi och bioteknologi, Sveriges lantbruksuniversitet

        UKÄ forskningsämne

        Polymerkemi
        Analytisk kemi
        Organisk kemi

        Publikationens identifierare

        DOI: https://doi.org/10.1016/j.carbpol.2016.11.029

        Permanent länk till denna sida (URI)

        https://res.slu.se/id/publ/88732