Sammanfattning

Hematopoietic serine proteases (SPs) are stored in the granules of different leukocytes and these enzymes are important effector molecules in the immune system of mammals. However, very little is known about the presence of these proteins in lower vertebrates. Herein, the primary structures of five novel fish SPs, from the Atlantic cod (Gadus morhua) and the channel catfish (Ictalurus punctatus), are presented. One of the cod SPs is a homologue to human GzmA and K. The other fish SPs identified are termed 'Gzm-like' and are distantly related to a large heterogeneous group of hematopoietic SPs, including most of the T-cell Gzms (B-H), the mast cell chymases, the mast cell/basophil proteases of the mouse mast cell protease-8 subfamily (W-family) and the neutrophil cathepsin G. Extensive BLAST-searches in genome and expressed sequence tag (EST) databases identified 40 additional teleost SPs related to the mammalian hematopoietic SP family. Subsequent phylogenetical analyses clearly demonstrate that the diversification into different subgroups within the GzmB/chymase/cathepsin G-related family has occurred independently in bony fishes and in mammals. In contrast, our findings suggest that the three subgroups, including (1) GzmK and the potent apoptosis-inducing GzmA, (2) the neutrophil proteases (proteinase 3, N-elastase and azurocidin), and (3) adipsin, have all evolved as distinct groups before the separation of tetrapods from the ray-finned fish approximately 420 million years ago. (c) 2005 Elsevier Ltd. All rights reserved

Publicerad i

Developmental and Comparative Immunology
2006, Volym: 30, nummer: 10, sidor: 901-918
Utgivare: PERGAMON-ELSEVIER SCIENCE LTD

SLU författare

• Wernersson, Sara

  • Institutionen för molekylär biovetenskap, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Veterinärmedicin
Husdjursvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.dci.2005.10.014

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/9050