Sammanfattning

Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.

Nyckelord

Arabidopsis thaliana; Micro-grafting; Chimeric plants; Mobile molecules; Graft-transmissible signal

Publicerad i

Methods in Molecular Biology
2017, Volym: 1497, sidor: 9-18
Titel: Plant Hormones: Methods and Protocols
ISBN: 978-1-4939-6469-7, eISBN: 978-1-4939-6467-3
Utgivare: Humana Press

UKÄ forskningsämne

Utvecklingsbiologi


Publikationens identifierare

DOI: https://doi.org/10.1007/978-1-4939-6469-7_2

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/94466