Bokkapitel2017Vetenskapligt granskad
Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis thaliana
Melnyk, Charles W.
Sammanfattning
Plants have a remarkable ability to regenerate vascular tissue after damage or wounding. A striking example of this phenomenon is the cutting and rejoining of plants during the process of grafting, which humans have used for millennia. Here, I describe how to graft Arabidopsis seedlings and how to monitor the vascular reconnection process during wound healing using fluorescent dyes and fluorescent proteins. Furthermore, I describe how to visualize xylem formation during graft healing. These techniques are useful for studying how the vascular system regenerates and for better understanding the process of plant grafting.
Nyckelord
Arabidopsis thaliana; Micro-grafting; Chimeric plants; Fluorescent dyes; Vascular regeneration; Xylem formation
Publicerad i
Methods in Molecular Biology
2017, Volym: 1544, nummer: 1544, sidor: 91-102
Titel: Xylem: Methods and Protocols
ISBN: 978-1-4939-6722-3, eISBN: 978-1-4939-6720-9
Utgivare: Springer
UKÄ forskningsämne
Utvecklingsbiologi
Publikationens identifierare
DOI: https://doi.org/10.1007/978-1-4939-6722-3_9
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/94468