Sammanfattning

Polypeptides from animal venoms have found important uses as drugs, pharmacological tools, and within biotechnological and agricultural applications. We here report a novel family of cystine knot peptides from nemertean worms, with potent activity on voltage-gated sodium channels. These toxins, named the alpha-nemertides, were discovered in the epidermal mucus of Lineus longissimus, the 'bootlace worm' known as the longest animal on earth. The most abundant peptide, the 31-residue long alpha-1, was isolated, synthesized, and its 3D NMR structure determined. Transcriptome analysis including 17 species revealed eight alpha-nemertides, mainly distributed in the genus Lineus. alpha-1 caused paralysis and death in green crabs (Carcinus maenas) at 1 mu g/kg (similar to 300 pmol/kg). It showed profound effect on invertebrate voltage-gated sodium channels (e.g. Blattella germanica Na(v)1) at low nanomolar concentrations. Strong selectivity for insect over human sodium channels indicates that a-nemertides can be promising candidates for development of bioinsecticidal agents.

Publicerad i

Scientific Reports
2018, Volym: 8, artikelnummer: 4596
Utgivare: NATURE PUBLISHING GROUP

SLU författare

• Strand, Malin

  • SLU Artdatabanken, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Farmakologi och toxikologi


Publikationens identifierare

DOI: https://doi.org/10.1038/s41598-018-22305-w

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/94714