Forskningsartikel2018Vetenskapligt granskad
Complex effect of lignocellulosic biomass pretreatment with 1-butyl-3-methylimidazolium chloride ionic liquid on various aspects of ethanol and fumaric acid production by immobilized cells within SSF
Dotsenko, Anna S.; Dotsenko, Gleb S.; Senko, Olga V.; Stepanov, Nikolay A.; Lyagin, Ilya V.; Efremenko, Elena N.; Gusakov, Alexander V.; Zorov, Ivan N.; Rubtsova, Ekaterina A.
Sammanfattning
The pretreatment of softwood and hardwood samples (spruce and hornbeam wood) with 1-butyl-3-methylimidazolium chloride ([Bmim] Cl) was undertaken for further simultaneous enzymatic saccharification of renewable non-food lignocellulosic biomass and microbial fermentation of obtained sugars to ethanol and fumaric acid. A multienzyme cocktail based on cellulases and yeast or fungus cells producing ethanol and fumaric acid were the main objects of [Bmim] Cl influence studies. A complex effect of lignocellulosic biomass pretreatment with [Bmim] Cl on various aspects of the process (both action of cellulases and microbial conversion of hydrolysates to target products) was revealed. Positive effects of the pretreatment with [Bmim] Cl included decreasing the lignin content in the biomass, and increasing the effectiveness of enzymatic hydrolysis and microbial transformation of pretreated biomass. Immobilized cells of both yeasts and fungi possessed improved productive characteristics in the biotransformation of biomass pretreated with [Bmim] Cl to ethanol and fumaric acid.
Nyckelord
Imidazolium-based ionic liquid [Bmim]Cl; Pretreatment of biomass; Simultaneous enzymatic saccharification and microbial fermentation (SSF); Immobilized cells; Cryogel of polyvinyl alcohol
Publicerad i
Bioresource Technology
2018, Volym: 250, sidor: 429-438
Utgivare: ELSEVIER SCI LTD
UKÄ forskningsämne
Biokatalys och enzymteknik
Publikationens identifierare
DOI: https://doi.org/10.1016/j.biortech.2017.11.064
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/95128