Sammanfattning

Fourier transform infrared (FT-IR) spectroscopy is a fast, sensitive, inexpensive, and nondestructive technique for chemical profiling of plant materials. In this chapter we discuss the instrumental setup, the basic principles of analysis, and the possibilities for and limitations of obtaining qualitative and semiquantitative information by FT-IR spectroscopy. We provide detailed protocols for four fully customizable techniques: (1) Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS): a sensitive and high-throughput technique for powders; (2) attenuated total reflectance (ATR) spectroscopy: a technique that requires no sample preparation and can be used for solid samples as well as for cell cultures; (3) microspectroscopy using a single element (SE) detector: a technique used for analyzing sections at low spatial resolution; and (4) microspectroscopy using a focal plane array (FPA) detector: a technique for rapid chemical profiling of plant sections at cellular resolution. Sample preparation, measurement, and data analysis steps are listed for each of the techniques to help the user collect the best quality spectra and prepare them for subsequent multivariate analysis.

Nyckelord

Fourier transform infrared spectroscopy; Methods; Microspectroscopy; Chemical composition; Multivariate analysis; Plant; Attenuated total reflectance; Diffuse reflectance; Focal plane array detector

Publicerad i

Methods in Molecular Biology
2014, Volym: 1062, nummer: 1062, sidor: 317-352
Titel: Arabidopsis Protocols
ISBN: 978-1-62703-579-8, eISBN: 978-1-62703-580-4
Utgivare: Humana Press

SLU författare

• Sundberg, Björn

  • Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Biokemi och molekylärbiologi


Publikationens identifierare

DOI: https://doi.org/10.1007/978-1-62703-580-4_18

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/95240