Sammanfattning

Evans blue dye disappears slowly from blood plasma and is not ideal for repeated measurements of plasma volume in the same goat because of increasing turbulence of the plasma. Therefore, indocyanine green (ICG) was evaluated as an alternative to Evans blue in goats. The plasma volume was 40 +/- 6 ml kg(-1) and 41 +/- 3 ml kg(-1) measured with ICG and Evans blue, respectively, in six non-pregnant, non-lactating goats. In three other goats plasma volume was measured during late lactation (7-8 months after parturition; milk production 1.0 +/- 0.6 l). It was 52 +/- 1 ml kg(-1) (ICG) and 51 +/- 5 ml kg(-1) (Evans blue), respectively. Thus, values of plasma volume measured by ICG corresponded well to these achieved with Evans blue. In a second series ICG was used repeatedly in the same goats to measure plasma volumes during late pregnancy and early lactation. A group of non-pregnant, non-lactating goats was used as control. Plasma volume was 39 +/- 3 ml kg(-1) (N = 7) and 44 +/- 8 ml kg(-1) (N = 6) at 23 +/- 7 days and 2.5 +/- 1 days before parturition, respectively. During early lactation it was 70 +/- 12 ml kg(-1) (23 +/- 5 days after parturition; milk production 2.4 +/- 0.5 l N = 7). No side effects were observed after several injections of ICG in any of the goats. In the control group, plasma volume was 40 +/- 7 ml kg(-1), which was the same value as that observed in the first series. We conclude that ICG is a good alternative to Evans blue for determination of plasma volume in goats.

Nyckelord

indocyanine green; goat; plasma volume; pregnancy; lactation

Publicerad i

Small Ruminant Research
1996, Volym: 19, nummer: 1, sidor: 8683
Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Sandberg, Eva

  • Institutionen för djurfysiologi, Sveriges lantbruksuniversitet
    

• Dahlborn, Kristina

  • Institutionen för djurfysiologi, Sveriges lantbruksuniversitet
    

• Olsson, Kerstin

  • Institutionen för djurfysiologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Klinisk vetenskap
Medicinsk biovetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/0921-4488(95)00730-X

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/95597