Belak, Sandor
- Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
Detection of caliciviruses requires high mutation tolerance and throughput. The development of a rational simple, single tube reverse transcription-real-time quantitative PCR (QPCR) technique for human noroviruses (NV) is reported here. A dual-probe, triple-primer system (NM system) was used for simultaneous detection and preliminary differentiation of NV genogroups in fecal samples. The design was based on a comprehensive analysis of all 1140 NV sequences available in GenBank. A touch-down amplification protocol improved the frequency of detection. The final QPCR was evaluated with 71 fecal samples from outbreak and sporadic cases in Sweden (1997-2004), all calicivirus-positive by electron microscopy. Up to 56 (79%) were positive. The method is more rational than NV detection methods described previously, and should be a developmental basis for large-scale routine methods for detection of NV. (c) 2005 Elsevier B.V. All fights reserved.
Nyckelord
norovirus; real-time; PCR; QPCR; gastroenteritis; virus quantitation
Publicerad i
Journal of Virological Methods
2006, volym: 132, nummer: 45659, sidor: 69-76
Utgivare: ELSEVIER SCIENCE BV
SLU författare
UKÄ forskningsämne
Mikrobiologi inom det medicinska området
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.jviromet.2005.09.006
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/10088