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Sammanfattning

Cryopreservation is stressful to sperm cells inducing an increase in the production of reactive oxygen species and subsequently reducing post-thaw sperm quality. With the present study, there was evaluation of the protective effects of two antioxidants, epigallocatechin (1 mM) and catalase (500 IU/ml), added at thawing, as well as inter-individual variation on quality of cryopreserved dromedary camel spermatozoa. Semen was collected from six males and sperm, selected using single layer centrifugation, were cryopreserved. Post-thaw sperm quality was evaluated by assessing motility variables, viability and acrosome integrity then sperm were co-incubated with or without antioxidant (control) and further assessed at 1.5 and 3 h of the incubation period. Oxidative damage was measured colorimetrically for malondialdehyde production at 3 h of the incubation period. With the use of epigallocatechin there were not promising results, however, with use of catalase there were greater total and progressive motility, and values for some kinematic variables (P

Nyckelord

Dromedary camel; Sperm; Cryopreservation; Catalase; Epigallocatechin

Publicerad i

Animal Reproduction Science
2019, volym: 209, artikelnummer: 106168
Utgivare: ELSEVIER

SLU författare

UKÄ forskningsämne

Klinisk vetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1016/j.anireprosci.2019.106168

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/103183