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Sammanfattning

PSP-I/PSP-II heterodimer is a major protein of boar seminal plasma which is able to preserve, in vitro, the viability, motility, and mitochondrial activity of highly extended boar spermatozoa for at least 5 hours. However, little is known about the binding pattern of the heterodimer to the sperm plasma membrane and its eventual relation with the maintenance of the sperm functionality. The present study investigated the effect of exposing highly extended boar spermatozoa (11 million/mL) to 1.5 mg/mL of PSP-I/PSP-II for 0.5, 5, and 10 hours at 38 degrees C on sperm characteristics and the changes in PSP-I/PSP-II localization as a result of both the addition of PSP-I/PSP-II to the extender and the incubation time. Exposure of the spermatozoa to PSP-I/PSP-II preserved sperm viability, motility, and mitochondrial activity when compared to nonexposed spermatozoa. This protective effect lasted for 10 hours (P

Nyckelord

reproductive tract; semen; sperm; seminal plasma; sperm capacitation

Publicerad i

Journal of Andrology
2006, volym: 27, nummer: 6, sidor: 766-773

SLU författare

UKÄ forskningsämne

Husdjursvetenskap
Veterinärmedicin

Publikationens identifierare

  • DOI: https://doi.org/10.2164/jandrol.106.000539

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/10398