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Sammanfattning

Thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK) are required for the activation of thymidine and deoxycytidine analogs used in antiviral and anticancer therapies. Many anticancer drugs cause oxidative stress, and the rise of GSSG and other reactive oxygen species may lead to alteration in gene expression, protein, nucleic acids and lipid modifications. Here, we investigated the effects of oxidative stress and nucleoside analog on the expression and activity of TK1 and dCK. Treatment with GSSG resulted in glutathionylation of dCK and dGK but not TK1 and Dm-dNK, and glutathionylation led to increased dCK activity but decreased dGK activity. Treatment with hydrogen peroxide resulted in induction of TK1, however, the TK1 activity did not correlate with TK1 protein levels, indicating that TK1 protein was inactive. The cellular expression of dCK, however, was reduced but dCK activity was not affected at concentration

Nyckelord

Deoxycytidine kinase; doxorubicin; nucleoside analogs; oxidative stress; thymidine kinase 1

Publicerad i

Nucleosides, Nucleotides and Nucleic Acids
2020, volym: 39, nummer: 10-12, sidor: 1347-1358
Utgivare: TAYLOR & FRANCIS INC

SLU författare

Associerade SLU-program

AMR: Virus

Globala målen (SDG)

SDG3 God hälsa och välbefinnande

UKÄ forskningsämne

Biokemi
Molekylärbiologi

Publikationens identifierare

  • DOI: https://doi.org/10.1080/15257770.2020.1720234

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/105212