Fossum, Caroline
- Institutionen för veterinärmedicinsk mikrobiologi, Sveriges lantbruksuniversitet
Sammanfattning
This study aimed to evaluate the possibility to detect early changes in gut-associated lymphoid tissue related to an inflammatory response, Anaesthetised pigs were subjected to faecal peritonitis (n = 9) or to a sham procedure (n = 8), Blood from the vena cava and the superior mesenteric vein was repeatedly sampled, and the levels of interleukin-6 (IL-6) were analysed, Biopsies of the small intestine and mesenteric lymph nodes (MLNs), harvested at 300 min, were incubated with monoclonal antibodies specific for CD2 (T lymphocytes), IgM (B lymphocytes) and CD11a/CD18 (leucocyte adhesion molecule). The number of positive (+) cells was scored, During peritonitis, IL-6 increased significantly. Compared to controls, the number of CD2+ cells decreased, IgM+ cells tended to increase and CD11a/CD18+ cells increased in the mucosa during peritonitis, In MLNs, the number of cells positive for all studied markers increased during peritonitis. We conclude that peritonitis causes an inflammatory response in the gut reflected by changes in the distribution of immune cells in gut-associated lymphoid tissue and release of IL-6 to venous blood. Copyright (C) 2001 S. Karger AG, Basel.
Nyckelord
lymphocytes; CD11a/CD18; IL-6; gut mesenteric lymph nodes; hypoxia; peritonitis; pigs
Publicerad i
European Surgical Research
2000, volym: 32, nummer: 6, sidor: 323-330
Utgivare: KARGER
SLU författare
UKÄ forskningsämne
Kirurgi
Publikationens identifierare
- DOI: https://doi.org/10.1159/000052213
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/106594