Fossum, Caroline
- Institutionen för molekylär biovetenskap, Sveriges lantbruksuniversitet
Sammanfattning
The cytokine inducing capacity of the vaccine vector pcDNA3, a methylated form of the plasmid, and pcDNA3 encoding porcine interleukin (IL)-6 or granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in pigs, using a model with tissue chambers implanted subcutaneously. The production of interferon (IFN)-alpha, IFN-gamma, IL-6 and GM-CSF was studied at local (tissue chamber fluid (TCF)) and systemic (serum) levels during 3 days post-injection. All forms of the plasmid, except the methylated, induced a transient local production of IFN-alpha but no plasmid-induced production of IFN-gamma, GM-CSF or IL-6 could be detected after injection of the plasmids. The IFN-alpha response increased markedly at repeated injections of pcDNA3. This IFN-alpha inducing capacity of the plasmid is likely to affect immune responses at DNA vaccination of pigs. (C) 2002 Elsevier Science B.V. All rights reserved.
Nyckelord
pig; plasmid; immunostimulatory DNA; cytokines
Publicerad i
Veterinary Immunology and Immunopathology
2002, volym: 87, nummer: 1-2, artikelnummer: PII S0165-2427(02)00027-7
Utgivare: ELSEVIER SCIENCE BV
SLU författare
Fuxler, Lisbeth
- Sveriges lantbruksuniversitet, Sveriges lantbruksuniversitet
- Sveriges lantbruksuniversitet, Sveriges lantbruksuniversitet
Domeika, Kristina
- Sveriges lantbruksuniversitet, Sveriges lantbruksuniversitet
- Sveriges lantbruksuniversitet, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Patobiologi
Publikationens identifierare
- DOI: https://doi.org/10.1016/S0165-2427(02)00024-7
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/106619