Transcriptional responses in Parascaris univalens after in vitro exposure to ivermectin, pyrantel citrate and thiabendazole

Abstract

Background: Parascaris univalensis a pathogenic parasite of foals and yearlings worldwide. In recent years,Parascarisspp. worms have developed resistance to several of the commonly used anthelmintics, though currently the mechanisms behind this development are unknown. The aim of this study was to investigate the transcriptional responses in adultP. univalensworms afterin vitroexposure to different concentrations of three anthelmintic drugs, focusing on drug targets and drug metabolising pathways.Methods: Adult worms were collected from the intestines of two foals at slaughter. The foals were naturally infected and had never been treated with anthelmintics. Worms were incubated in cell culture media containing different concentrations of either ivermectin (10(-9) M, 10(-11) M, 10(-13) M), pyrantel citrate (10(-6) M, 10(-8) M, 10(-10) M), thiabendazole (10(-5) M, 10(-7) M, 10(-9) M) or without anthelmintics (control) at 37 degrees C for 24 h. After incubation, the viability of the worms was assessed and RNA extracted from the anterior region of 36 worms and sequenced on an Illumina NovaSeq 6000 system.Results: All worms were alive at the end of the incubation but showed varying degrees of viability depending on the drug and concentration used. Differential expression (Padj < 0.05 and log2 fold change >= 1 or

Keywords

Transcriptome; Anthelmintic resistance; RNA sequencing; Differential expression; lgc-37

Published in

Parasites and Vectors
2020, volume: 13, number: 1, article number: 342
Publisher: BMC

SLU Authors

• Martin, Frida

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Dube, Faruk

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Karlsson Lindsjö, Oskar

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Höglund, Johan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Bergström, Tomas

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Tyden, Eva

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Parasite

UKÄ Subject classification

Pathobiology

Publication identifier

• DOI: https://doi.org/10.1186/s13071-020-04212-0

Permanent link to this page (URI)

https://res.slu.se/id/publ/107159