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Sammanfattning

Background: Parascaris univalensis a pathogenic parasite of foals and yearlings worldwide. In recent years,Parascarisspp. worms have developed resistance to several of the commonly used anthelmintics, though currently the mechanisms behind this development are unknown. The aim of this study was to investigate the transcriptional responses in adultP. univalensworms afterin vitroexposure to different concentrations of three anthelmintic drugs, focusing on drug targets and drug metabolising pathways.Methods: Adult worms were collected from the intestines of two foals at slaughter. The foals were naturally infected and had never been treated with anthelmintics. Worms were incubated in cell culture media containing different concentrations of either ivermectin (10(-9) M, 10(-11) M, 10(-13) M), pyrantel citrate (10(-6) M, 10(-8) M, 10(-10) M), thiabendazole (10(-5) M, 10(-7) M, 10(-9) M) or without anthelmintics (control) at 37 degrees C for 24 h. After incubation, the viability of the worms was assessed and RNA extracted from the anterior region of 36 worms and sequenced on an Illumina NovaSeq 6000 system.Results: All worms were alive at the end of the incubation but showed varying degrees of viability depending on the drug and concentration used. Differential expression (Padj < 0.05 and log2 fold change >= 1 or

Nyckelord

Transcriptome; Anthelmintic resistance; RNA sequencing; Differential expression; lgc-37

Publicerad i

Parasites and Vectors
2020, volym: 13, nummer: 1, artikelnummer: 342
Utgivare: BMC

SLU författare

Associerade SLU-program

AMR: Parasiter

UKÄ forskningsämne

Patobiologi

Publikationens identifierare

  • DOI: https://doi.org/10.1186/s13071-020-04212-0

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/107159