Bhalerao, Rishikesh P.
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
- Beijing Forestry University
Sammanfattning
The interaction between the receptor-like kinase (RLK) FERONIA (FER) and the secreted peptide RAPID ALKALINIZATION FACTOR1 (RALF1) is vital for development and stress responses in Arabidopsis. Ligand-induced membrane dynamics affect the function of several RLKs, but the effects of the RALF1-FER interaction on the dynamics of FER and the ensuing effects on its functionality are poorly understood. Here, we show that RALF1 modulated the dynamics and partitioning of FER-GFP at the plasma membrane (PM). Moreover, FER was internalized by both clathrin-mediated endocytosis (CME) and clathrin-independent endocytosis (CIE) under steady-state conditions. After RALF1 treatment, FERGFP internalization was primarily enhanced via the CME pathway, raising FER-GFP levels in the vacuole. RALF1 treatment also modulated trafficking of other PM proteins, such as PIN2-GFP and BRI1-GFP, increasing their vacuolar levels by enhancing their internalization. Importantly, blocking CME attenuated RALF1-mediated root growth inhibition independently of RALF1-induced early signaling, suggesting that the RALF1 can also exert its effects via the CME pathway. These findings reveal that the RALF1-FER interaction modulates plant growth and development, and this might also involve endocytosis of PM proteins.
Nyckelord
RALF1; FERONIA; Endocytosis; Root growth; Arabidopsis
Publicerad i
Development
2020, volym: 147, nummer: 13, artikelnummer: dev189902
Utgivare: COMPANY BIOLOGISTS LTD
SLU författare
UKÄ forskningsämne
Utvecklingsbiologi
Publikationens identifierare
- DOI: https://doi.org/10.1242/dev.189902
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/108016