A method to quantify the allelopathic compound batatasin-III in extracts from Empetrum hermaphroditum using gas chromatography: Applied on extracts from leaves of different ages

Sammanfattning

A gas chromatographic method was developed to quantify the allelopathic compound, batatasin-III, in organic and water extracts of leaves from the boreal dwarf shrub Empetrum hermaphroditum. The method provides a rapid and inexpensive quantification of small masses of batatasin-III in complex matrices. The compound was effectively separated from interfering compounds using solid-phase extraction techniques and chromatographed as a TMS-derivate on a nonpolar column. Batatasin-III was quantified in leaves and found to vary with the age of the shoot. The concentration of batatasin-III was highest in first- and second-year shoots, while third-year shoots contained about half that of younger shoots. Senescent leaves on older shoots still contained a considerable amount of batatasin-III. The method described provides an important tool for the demonstration of allelopathy.

Nyckelord

allelopathy; batatasin-III; quantification; gas chromatographic method; SPE techniques; Empetrum hermaphroditum

Publicerad i

Journal of Chemical Ecology
1997, volym: 23, nummer: 10, sidor: 2345-2355
Utgivare: SPRINGER

SLU författare

• Nilsson Hegethorn, Marie-Charlotte

  • Institutionen för skoglig vegetationsekologi, Sveriges lantbruksuniversitet
    

• Zackrisson, Olle

  • Institutionen för skoglig vegetationsekologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Ekologi

Publikationens identifierare

• DOI: https://doi.org/10.1023/B:JOEC.0000006678.37973.be

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/115264