Ortiz Rios, Rodomiro Octavio
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
Sammanfattning
Characterization of major resistance (R) genes to late blight (LB) -caused by the oomycete Phytophthora infestans- is very important for potato breeding. The objective of this study was to identify novel genes for resistance to LB from diploid Solanum tuberosum L. Andigenum Group (StAG) cultivar accessions. Using comparative analysis with a edgeR bioconductor package for differential expression analysis of transcriptomes, two of these accessions with contrasting levels of resistance to LB were analyzed using digital gene expression data. As a result, various differentially expressed genes (P = 0.0001, Log(2)FC = 2, FDR < 0.001) were noted. The combination of transcriptomic analysis provided 303 candidate genes that are overexpressed and underexpressed, thereby giving high resistance to LB. The functional analysis showed differential expression of R genes and their corresponding proteins related to disease resistance, NBS-LRR domain proteins, and specific disease resistance proteins. Comparative analysis of specific tissue transcriptomes in resistant and susceptible genotypes can be used for rapidly identifying candidate R genes, thus adding novel genes from diploid StAG cultivar accessions for host plant resistance to P. infestans in potato.
Nyckelord
gene expression; genome; host plant resistance; Phytophthora infestans; Solanum tuberosum
Publicerad i
Frontiers in Plant Science
2023, volym: 14, artikelnummer: 1210046
SLU författare
Associerade SLU-program
SLU Nätverk växtskydd
UKÄ forskningsämne
Trädgårdsvetenskap/hortikultur
Genetik och förädling inom lantbruksvetenskap
Jordbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.3389/fpls.2023.1210046
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/123937