Johannisson, Anders
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Uppsala universitet
Sammanfattning
The aim of the present study was to test a rapid, robust flow cytometric technique for the detection of sperm head abnormalities of domestic bulls and stallions. The so-called PulSA approach detects the pulse profiles of propidium-iodide labelled spermatozoa. In the first experiment, species-specific threshold values were established on sperm samples that were tested for sperm head abnormalities with a classic visual morphology analysis. In the second experiment, serial mixtures of bull and stallion spermatozoa mimicking different percentages of sperm head abnormalities were analysed. Non-metric multidimensional scaling showed a clear separation between the normal and mixed samples. The PulSA approach may be a useful tool in identifying sub- or infertile breeding males as well as in studying the evolutionary aspects of sperm morphology and morphometry.
Nyckelord
abnormalities; bull; PulSA; sperm quality; stallion
Publicerad i
Reproduction in Domestic Animals
2023, volym: 58, nummer: 11, sidor: 1569-1575
Utgivare: WILEY
SLU författare
UKÄ forskningsämne
Klinisk vetenskap
Husdjursvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1111/rda.14472
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/126499