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Abstract

In this study, the relationships between post-thaw bull sperm characteristics and hyperketonemic conditions after coincubation with cow plasma or media were determined to investigate if such a condition could affect bull sperm characteristics. Two experiments were conducted. In experiment 1, blood samples were collected from 31 cows to prepare plasma. Cows were independently categorized into two groups according to plasma beta-hydroxybutyrate (BHB) concentrations (above or below 1.2 mM). Thawed bull semen was diluted and incubated with diluted plasma; motility parameters were evaluated using Computer Assisted Semen Analysis (CASA). In experiment 2, a pooled sample of thawed semen was diluted and divided into three aliquots: without BHB (control) and treated with either 1.2 mM (1.2) or 3 mM (3) BHB. In addition to motility, flow cytometric analyses were carried out. In experiment 1, the overall motility decreased significantly in plasma containing high (>= 1.2 mM) BHB compared to plasma containing low (

Keywords

bovine; coincubation; hyperketonaemia; semen characteristics

Published in

Reproduction in Domestic Animals
2023, volume: 58, number: 12, pages: 1732-1744
Publisher: WILEY

SLU Authors

UKÄ Subject classification

Clinical Science

Publication identifier

  • DOI: https://doi.org/10.1111/rda.14492

Permanent link to this page (URI)

https://res.slu.se/id/publ/126889