Johannisson, Anders
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Sammanfattning
In this study, the relationships between post-thaw bull sperm characteristics and hyperketonemic conditions after coincubation with cow plasma or media were determined to investigate if such a condition could affect bull sperm characteristics. Two experiments were conducted. In experiment 1, blood samples were collected from 31 cows to prepare plasma. Cows were independently categorized into two groups according to plasma beta-hydroxybutyrate (BHB) concentrations (above or below 1.2 mM). Thawed bull semen was diluted and incubated with diluted plasma; motility parameters were evaluated using Computer Assisted Semen Analysis (CASA). In experiment 2, a pooled sample of thawed semen was diluted and divided into three aliquots: without BHB (control) and treated with either 1.2 mM (1.2) or 3 mM (3) BHB. In addition to motility, flow cytometric analyses were carried out. In experiment 1, the overall motility decreased significantly in plasma containing high (>= 1.2 mM) BHB compared to plasma containing low (
Nyckelord
bovine; coincubation; hyperketonaemia; semen characteristics
Publicerad i
Reproduction in Domestic Animals
2023, volym: 58, nummer: 12, sidor: 1732-1744
Utgivare: WILEY
SLU författare
Morrell, Jane
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Klinisk vetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1111/rda.14492
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/126889