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Research article2018Peer reviewedOpen access

S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants

Zhan, Ni; Wang, Chun; Chen, Lichao; Yang, Huanjie; Feng, Jian; Gong, Xinqi; Ren, Bo; Wu, Rong; Mu, Jinye; Li, Yansha; Liu, Zhonghua; Zhou, Ying; Peng, Juli; Wang, Kejian; Huang, Xun; Xiao, Shi; Zuo, Jianru

Abstract

Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy.

Published in

Molecular Cell
2018, volume: 71, number: 1, pages: 142-154

SLU Authors

  • Yang, Huanjie

    • Chinese Academy of Sciences
    • University of Chinese Academy of Sciences

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication identifier

  • DOI: https://doi.org/10.1016/j.molcel.2018.05.024

Permanent link to this page (URI)

https://res.slu.se/id/publ/127251