S-Nitrosylation Targets GSNO Reductase for Selective Autophagy during Hypoxia Responses in Plants

Sammanfattning

Nitric oxide (NO) regulates diverse cellular signaling through S-nitrosylation of specific Cys residues of target proteins. The intracellular level of S-nitrosoglutathione (GSNO), a major bioactive NO species, is regulated by GSNO reductase (GSNOR), a highly conserved master regulator of NO signaling. However, little is known about how the activity of GSNOR is regulated. Here, we show that S-nitrosylation induces selective autophagy of Arabidopsis GSNOR1 during hypoxia responses. S-nitrosylation of GSNOR1 at Cys-10 induces conformational changes, exposing its AUTOPHAGY-RELATED8 (ATG8)-interacting motif (AIM) accessible by autophagy machinery. Upon binding by ATG8, GSNOR1 is recruited into the autophagosome and degraded in an AIM-dependent manner. Physiologically, the S-nitrosylation-induced selective autophagy of GSNOR1 is relevant to hypoxia responses. Our discovery reveals a unique mechanism by which S-nitrosylation mediates selective autophagy of GSNOR1, thereby establishing a molecular link between NO signaling and autophagy.

Publicerad i

Molecular Cell
2018, volym: 71, nummer: 1, sidor: 142-154

SLU författare

• Yang, Huanjie

  • Chinese Academy of Sciences
  • University of Chinese Academy of Sciences

UKÄ forskningsämne

Biokemi och molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.molcel.2018.05.024

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/127251