Bhalerao, Rishikesh P.
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
Sammanfattning
Hydrolyzable tannins (HTs), predominant polyphenols in oaks, are widely used in grape wine aging, feed additives, and human healthcare. However, the limited availability of a high-quality reference genome of oaks greatly hampered the recognition of the mechanism of HT biosynthesis. Here, high-quality reference genomes of three Asian oak species (Quercus variabilis, Quercus aliena, and Quercus dentata) that have different HT contents were generated. Multi-omics studies were carried out to identify key genes regulating HT biosynthesis. In vitro enzyme activity assay was also conducted. Dual-luciferase and yeast one-hybrid assays were used to reveal the transcriptional regulation. Our results revealed that beta-glucogallin was a biochemical marker for HT production in the cupules of the three Asian oaks. UGT84A13 was confirmed as the key enzyme for beta-glucogallin biosynthesis. The differential expression of UGT84A13, rather than enzyme activity, was the main reason for different beta-glucogallin and HT accumulation. Notably, sequence variations in UGT84A13 promoters led to different trans-activating activities of WRKY32/59, explaining the different expression patterns of UGT84A13 among the three species. Our findings provide three high-quality new reference genomes for oak trees and give new insights into different transcriptional regulation for understanding beta-glucogallin and HT biosynthesis in closely related oak species.
Nyckelord
hydrolyzable tannin; oak; whole-genome sequencing; beta-glucogallin
Publicerad i
New Phytologist
2024, volym: 242, nummer: 6, sidor: 2702-2718
Utgivare: WILEY
SLU författare
UKÄ forskningsämne
Genetik och genomik
Botanik
Publikationens identifierare
- DOI: https://doi.org/10.1111/nph.19711
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/129272