1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium

Sammanfattning

A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium (PcCel45A) was expressed in Pichia pastoris with C-13 and N-15 labelling. A nearly complete assignment of H-1, C-13 and 15N backbone resonances was obtained, as well as the secondary structure prediction based on the assigned chemical shifts using the TALOS-N software. The predicted secondary structure was almost identical to previously published crystal structures of the same enzyme, except for differences in the termini of the sequence. This is the first NMR study using an isotopically labelled GH45 enzyme.

Nyckelord

PcCel45A; Cel45A; GH45; Endoglucanase; NMR

Publicerad i

Biomolecular NMR Assignments
2024, volym: 18, nummer: 2, sidor: 153-157
Utgivare: SPRINGER

SLU författare

• Okmane, Laura

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Sandgren, Mats

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Ståhlberg, Jerry

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Nestor, Gustav

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Strukturbiologi
Biokemi och molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1007/s12104-024-10182-6

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/131086