Johannisson, Anders
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Uppsala University
Abstract
Not all boar sperm samples survive cryopreservation well. A method of eliminating damaged sperm might enable more cryopreserved boar semen to be used for pig breeding. In this study we investigated the use of Magnetic Activated Cell sorting (MACS) to eliminate damaged sperm from thawed boar semen samples. The thawed samples were mixed with Dead cell removal particles and were applied to the column in a SuperMACS II. Different fractions were collected: Original sample (O), Flow-through (FT), and Eluate (E). Sperm membrane integrity, mitochondrial membrane potential and reactive oxygen species were evaluated by flow cytometry after staining with SYBR 14 and propidium iodide, or 5 ', 6, 6 '-tetrachloro-1, 1 ', 3, 3 '-tetraethylbenzimidazolylcarbocyanine iodide, or hydroethidine and dichlorodihydrofluorescein diacetate, respectively. The FT samples had increased membrane integrity, a greater proportion of sperm with high mitochondrial membrane potential and a greater proportion of sperm negative for hydrogen peroxide than O samples (P
Keywords
MACS; Cryopreserved boar semen; Sperm membrane damage; Apoptosis; Reactive Oxygen Species; Reproductive biotechnologies
Published in
Animal Reproduction Science
2024, volume: 256, article number: 107493
SLU Authors
Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
Wallgren, Margareta
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Clinical Science
Publication identifier
- DOI: https://doi.org/10.1016/j.anireprosci.2024.107493
Permanent link to this page (URI)
https://res.slu.se/id/publ/131696