Johannisson, Anders
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Uppsala universitet
Sammanfattning
Not all boar sperm samples survive cryopreservation well. A method of eliminating damaged sperm might enable more cryopreserved boar semen to be used for pig breeding. In this study we investigated the use of Magnetic Activated Cell sorting (MACS) to eliminate damaged sperm from thawed boar semen samples. The thawed samples were mixed with Dead cell removal particles and were applied to the column in a SuperMACS II. Different fractions were collected: Original sample (O), Flow-through (FT), and Eluate (E). Sperm membrane integrity, mitochondrial membrane potential and reactive oxygen species were evaluated by flow cytometry after staining with SYBR 14 and propidium iodide, or 5 ', 6, 6 '-tetrachloro-1, 1 ', 3, 3 '-tetraethylbenzimidazolylcarbocyanine iodide, or hydroethidine and dichlorodihydrofluorescein diacetate, respectively. The FT samples had increased membrane integrity, a greater proportion of sperm with high mitochondrial membrane potential and a greater proportion of sperm negative for hydrogen peroxide than O samples (P
Nyckelord
MACS; Cryopreserved boar semen; Sperm membrane damage; Apoptosis; Reactive Oxygen Species; Reproductive biotechnologies
Publicerad i
Animal Reproduction Science
2024, volym: 256, artikelnummer: 107493
SLU författare
Morrell, Jane
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
Wallgren, Margareta
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
- Institutionen för kliniska vetenskaper, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Klinisk vetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.anireprosci.2024.107493
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/131696