Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states

Sammanfattning

Eukaryotes can have thousands of 45S ribosomal RNA (rRNA) genes, many of which are silenced during development. Using fluorescence-activated sorting techniques, we show that active rRNA genes in Arabidopsis thaliana are present within sorted nucleoli, whereas silenced rRNA genes are excluded. DNA methyltransferase (met1), histone deacetylase (hda6), or chromatin assembly (caf1) mutants that disrupt silencing abrogate this nucleoplasmic-nucleolar partitioning. Bisulfite sequencing data indicate that active nucleolar rRNA genes are nearly completely demethylated at promoter CGs, whereas silenced genes are nearly fully methylated. Collectively, the data reveal that rRNA genes occupy distinct but changeable nuclear territories according to their epigenetic state. © 2013 by Cold Spring Harbor Laboratory Press.

Nyckelord

Chromatin assembly; DNA methylation; Gene silencing; Histone deacetylation; Ribosomal RNA gene; RNA polymerase I; Transcription

Publicerad i

Genes and Development
2013, volym: 27, sidor: 1545-1550

SLU författare

• Mozgova, Iva

  • Masarykova Univerzita

UKÄ forskningsämne

Genetik och genomik

Publikationens identifierare

• DOI: https://doi.org/10.1101/gad.221648.113

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/132191