Skip to main content
SLU:s publikationsdatabas (SLUpub)

Sammanfattning

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs. © Springer Science+Business Media, LLC 2013.

Nyckelord

Bioinformatics; High-throughput sequencing; MicroRNA; Northern blot; Small RNA

Publicerad i

Methods in Molecular Biology
2013, volym: 983, nummer: 983, sidor: 125-138
Titel: Dictyostelium discoideum Protocols
Utgivare: Humana Press Inc.

SLU författare

  • Avesson, Lotta

    • Garvan Institute of Medical Research
    • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet

  • Söderbom, Fredrik

    • Institutionen för molekylärbiologi, Sveriges lantbruksuniversitet

UKÄ forskningsämne

Bioinformatik (beräkningsbiologi)
Biokemi
Molekylärbiologi

Publikationens identifierare

  • DOI: https://doi.org/10.1007/978-1-62703-302-2_7
  • ISBN: 9781627033015
  • eISBN: 978-1-62703-302-2

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/132195