Standard methods for European foulbrood research 2.0

Sammanfattning

European foulbrood (EFB) is a severe bacterial honey bee brood disease caused by the Gram-positive bacterium Melissocccus plutonius. The disease is widely distributed worldwide, and is an increasing problem in some areas. Although the causative agent of EFB was described almost a century ago, many basic aspects of its pathogenesis are still unknown. Earlier studies were hampered by insensitive and unspecific methods such as culture based techniques. Recent advances in molecular technologies have led to a boom in the methods available to study both disease and causative organism, but not all published methods offer data of equal quality, or are likely to result in success. This paper presents selected step-by-step methodologies that have been used with success in at least one laboratory of the authors and considered relevant by the consortium of authors. We hope this paper helps providing some assistance to those wishing to work on M. plutonius and EFB, and speeds up the discovery of new knowledge to improve the control of this damaging and often neglected disease.

Nyckelord

Honey bee; European foulbrood; Melissococcus plutonius; brood disease; methodologies; BEEBOOK; COLOSS

Publicerad i

Journal of Apicultural Research
2024
Utgivare: TAYLOR & FRANCIS LTD

SLU författare

• Forsgren, Eva

  • Institutionen för ekologi, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Zoologi

Publikationens identifierare

• DOI: https://doi.org/10.1080/00218839.2024.2380425

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/132403