Cattaneo, Alberto Maria
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
Forskningsartikel2025Vetenskapligt granskadÖppen tillgång
Functional Characterization of a Female-Biased Chemoreceptor of the Codling Moth (Cydia pomonella) Responding to Aldehydes and Other Volatile Compounds
Cattaneo, Alberto Maria; Kwadha, Charles A.; Pullmann-Lindsley, Heidi; Erdei, Anna L.; Pitts, R. Jason; Walker III, William B.
Sammanfattning
With the advent of semiochemical-based control strategies used to mitigate damage of agricultural pest moths, many studies have focused on the function of male-specific putative pheromone receptors (PRs). In this investigation, we instead isolated, heterologously expressed, and functionally characterized a female-biased candidate PR, CpomOR22, from the codling moth, Cydia pomonella. Using transgenic Drosophila melanogaster for single sensillum recording (SSR) and gas-chromatographic SSR, we tested both synthetic ligands and various apple headspace extracts, identifying saturated and unsaturated aldehydes (nonanal, decanal, undecanal, dodecanal; (Z)-4-undecenal and (Z)-6-undecenal) among the most active ligands. Parallel experiments expressing CpomOR22 in Xenopus oocytes confirmed the binding of nonanal, decanal and undecanal and revealed lactones (gamma-undecalactone and delta-dodecalactone) and several carboxylic acids as additional active compounds. The renowned ecological importance of aldehydes for the codling moth and the potential for newly identified ligands, such as lactones, may inform innovative control strategies based on novel semiochemicals to interfere with the female-specific chemosensory systems of this insect.
Nyckelord
Cydia pomonella chemoreceptors; Heterologous expression; Transgenic Drosophila melanogaster; Single sensillum recording (SSR); Gas-chromatography-coupled SSR (GC-SSR); Xenopus oocytes
Publicerad i
Journal of Chemical Ecology
2025, volym: 51, nummer: 2, artikelnummer: 28
Utgivare: SPRINGER
SLU författare
Kwadha, Charles
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
Erdei, Anna Laura
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
Walker, William
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
- United States Department of Agriculture (USDA)
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Biokemi
Jordbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1007/s10886-025-01579-1
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/140985