Adipogenic characterization of immortalized CD55+ progenitor cells from human white adipose tissue

Sammanfattning

Background Mature adipocytes are difficult to study ex vivo, prompting the use of human adipose progenitor cells (hAPCs). However, hAPCs undergo replicative senescence, limiting their utility in long-term studies. Methods We inserted human telomerase reverse transcriptase (TERT) into the AAVS1 locus of CD55+ hAPCs derived from abdominal subcutaneous adipose tissue, and characterized the cells before and after adipogenic differentiation. Results TERT-hAPCs retained proliferative and adipogenic capacities for over 80 passages, comparable to early-passage wild type hAPCs. Transcriptomic and proteomic analyses confirmed strong adipocyte gene expression. Functionally, TERT-hAPCs responded to insulin and lipolytic stimuli (isoprenaline, dibutyryl cAMP, TNF-α). They adapted well to both 2D and 3D cultures, with improved adipogenesis under spheroid conditions. Conclusion Immortalization of CD55+ hAPCs yields cells with stable proliferative and adipogenic capacity across passages. Being cryopreservable and suitable for both 2D and 3D cultures, TERT-hAPCs offer a reliable, reusable model system for adipocyte studies using cells with a consistent genetic background.

Nyckelord

Genetic engineering; CRISPR/Cas9; multiomics; spheroid; fat cell; adipocyte; cell model; adipogenesis; lipolysis

Publicerad i

Adipocyte
2025, volym: 14, nummer: 1, artikelnummer: 2283213

SLU författare

• Omar Hmeadi, Muhmmad

  • Karolinska institutet

UKÄ forskningsämne

Cell- och molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1080/21623945.2023.2283213

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/142064