Vetukuri, Ramesh
- Department of Plant Breeding, Swedish University of Agricultural Sciences
Abstract
RNA silencing is a core cellular process that acts to defend the genome against potentially damaging genetic elements such as viruses and transposons. It has been extensively characterized in many eukaryotes and exploited as a tool for determining gene function through removing the activity of specific genes. It has also been used in Phytophthora species to reveal genes involved in different lifecycle stages. In this chapter, we provide guidelines and outline considerations for carrying out RNA silencing experiments in Phytophthora.
Keywords
Gene expression; Heterochromatin; Phytophthora; RNA interference; RNA silencing; sRNA
Published in
Title: Phytophthora : Methods and Protocols
Publisher: Humana Press Inc.
SLU Authors
UKÄ Subject classification
Microbiology
Genetics and Genomics
Publication identifier
- DOI: https://doi.org/10.1007/978-1-0716-4330-3_2
- ISBN: 978-1-0716-4329-7
- eISBN: 978-1-0716-4330-3
Permanent link to this page (URI)
https://res.slu.se/id/publ/142905