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Sammanfattning

RNA silencing is a core cellular process that acts to defend the genome against potentially damaging genetic elements such as viruses and transposons. It has been extensively characterized in many eukaryotes and exploited as a tool for determining gene function through removing the activity of specific genes. It has also been used in Phytophthora species to reveal genes involved in different lifecycle stages. In this chapter, we provide guidelines and outline considerations for carrying out RNA silencing experiments in Phytophthora.

Nyckelord

Gene expression; Heterochromatin; Phytophthora; RNA interference; RNA silencing; sRNA

Publicerad i

Titel: Phytophthora : Methods and Protocols
Utgivare: Humana Press Inc.

SLU författare

UKÄ forskningsämne

Mikrobiologi
Genetik och genomik

Publikationens identifierare

  • DOI: https://doi.org/10.1007/978-1-0716-4330-3_2
  • ISBN: 978-1-0716-4329-7
  • eISBN: 978-1-0716-4330-3

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/142905