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Sammanfattning

Potato (Solanum tuberosum L.) yield is highly sensitive to drought stress, yet robust phenotyping methods for drought tolerance remain scarce. To address this challenge, we present a rapid, high-throughput hydroponic assay designed as an efficient pre-screening tool for evaluating potato cultivars and CRISPR-edited lines. This protocol serves as a foundational screen, enabling researchers to identify the most promising genotypes before committing resources to more extensive soil or field trials. The system uses repurposed pipette tip boxes as low-cost, scalable hydroponic units, making the method highly accessible. Over a five-week period, plantlets are subjected to controlled 24-hr osmotic stress with polyethylene glycol (PEG-6000) and then assessed for resilience based on biomass, photosynthetic measurements, and visual recovery. This resource-efficient assay provides a controlled environment to minimize experimental noise and has been successfully applied to characterize CRISPR-edited potato lines. By providing a reproducible platform for initial evaluation, this protocol accelerates the selection pipeline for developing robust potato varieties for a changing climate. (c) 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Preparation of stem explants and in vitro rootingBasic Protocol 2: Assembly and maintenance of tip-box hydroponic unitsBasic Protocol 3: PEG-induced drought treatment and recoveryBasic Protocol 4: Post-stress phenotypic, biomass, and photosynthetic analysis

Nyckelord

abiotic stress screening; CRISPR; drought tolerance; high-throughput phenotyping; potato (Solanum tuberosum)

Publicerad i

Current protocols
2025, volym: 5, nummer: 7, artikelnummer: e70180
Utgivare: WILEY

SLU författare

UKÄ forskningsämne

Genetik och förädling inom lantbruksvetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1002/cpz1.70180

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/143201