Yong, Jean W.H
- Institutionen för biosystem och teknologi, Sveriges lantbruksuniversitet
Sammanfattning
This study aimed to develop a cost-effective and efficient enzymatic cocktail from spent mushroom substrate (SMS) while serving as a model to understand fungal-fermentative communities and characterize microbial diversity stability in SMS valorization. Co-cultivation of Aspergillus fumigatus IMCC2006 and Trichoderma asperellum IMCC2012 was optimized for cellulase production under varying moisture, pH, and fermentation time. Optimal enzyme yields were achieved at 60 % moisture, pH 5, and 96 h of fermentation, producing maximum CMCase (11.5 U g− 1 ) and FPase (4.35 U g− 1 ) activities, while the highest xylanase activity (13.8 U g− 1 ) occurred at 70 % moisture. Scanning electron microscopy and FTIR confirmed the consortium’s cellulolytic capability in SMS degradation. Microbial community analysis revealed Proteobacteria and Firmicutes dominance at the end of fermentation, supporting system stability. The findings provided a plausible solution for valorization of agricultural residues for enhanced enzymatic production from the synergistic consortium, to catalyze biorefinery applications.
Nyckelord
Spent mushroom substrate; Enzymatic cocktail, solid-state fermentation; Cellulase; Bacterial community diversity
Publicerad i
Bioresource technology reports
2025, volym: 32, artikelnummer: 102417
SLU författare
Globala målen (SDG)
SDG9 Hållbar industri, innovationer och infrastruktur
SDG12 Hållbar konsumtion och produktion
UKÄ forskningsämne
Mikrobiologi
Annan miljöbioteknik
Biokatalys och enzymteknik
Publikationens identifierare
- DOI: https://doi.org/10.1016/j.biteb.2025.102417
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/144663