Dotson, Bradley
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
- Lunds Universitet
Sammanfattning
Plant-symbiotic Trichoderma fungi attack microorganisms by secreting antibiotic membrane-permeabilising peptaibols such as alamethicin. These peptaibols also permeabilise plant root epidermis plasma membranes (PMs), but mild pretreatment with Trichoderma cellulase activates a unique cellulase-induced resistance to alamethicin (CIRA), via an unknown mechanism. We identify two Arabidopsis genes that are essential for the CIRA process: CIRA12 encodes a phosphatidylserine (PS) decarboxylase and CIRA13, a phospholipase D zeta, implying that specific changes in anionic membrane lipids mediate alamethicin resistance. Fluorescent sensors revealed that cellulase induced a laterally asymmetric decrease in PS and surface charge to outer periclinal root epidermal PMs. Consistently, the CIRA response was reversed by addition of lysoPS. CIRA13 is essential for vesicle trafficking, which in turn is crucial for CIRA induction. Overall, cellulase induces a cellular polarity with respect to phospholipids, not previously observed in plants, that is leading to increased lipid packing and preventing peptaibol permeabilization of the outer periclinal membrane.
Nyckelord
alamethicin; Arabidopsis; cellulase; phosphatidic acid; phosphatidylserine; PHOSPHATIDYLSERINE DECARBOXYLASE3; PHOSPHOLIPASE D zeta 2; Trichoderma
Publicerad i
New Phytologist
2025
Utgivare: WILEY
SLU författare
Lager, Ida
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
UKÄ forskningsämne
Genetik och förädling inom lantbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1111/nph.70721
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/144784