Khomutovska, Nataliia
- Institutionen för växtskyddsbiologi, Sveriges lantbruksuniversitet
Sammanfattning
Extreme environments are a largely unexplored reservoir of microbial diversity, with a remarkable potential to be exploited in agriculture. One hundred and seventeen yeast isolates, derived from different ecosystems in Italy, Sweden, Algeria, and France, were molecularly identified, and the most represented genus was Aureobasidium (57%). A phylogenetic analysis based on a multi-locus sequence typing (ITS, ELO, EF-1alpha) was conducted to characterize the black yeasts' population. To investigate A. pullulans extremophilic and extremotolerant behaviour, different temperatures and pH, together with the enzymatic production, were evaluated. The strains were tested by in vitro and in vivo assays against the postharvest fungal pathogen Monilinia fructicola as potential biocontrol agents (BCAs). Results displayed a great ecological variability concerning strains' growth and cell production depending on different culture conditions. However, a remarkable thermotolerance aptitude was detected in almost all the strains. In particular, the strains belonging to Group 2 (Algerian Desert) and 3 (Alto Adige Region) showed, respectively, higher thermotolerance and biocontrol ability. These findings showed how some extreme environments could represent a promising source for new potential BCAs. However, further studies are needed to investigate the mechanisms of action of these putative BCAs for application during the postharvest phase.
Nyckelord
cold environment; desert; urban sites; extremophiles; postharvest management
Publicerad i
Microorganisms
2025, volym: 13, nummer: 11, artikelnummer: 2596
Utgivare: MDPI
SLU författare
UKÄ forskningsämne
Genetik och förädling inom lantbruksvetenskap
Publikationens identifierare
- DOI: https://doi.org/10.3390/microorganisms13112596
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/144986