Uggla, Arvid
- Sveriges Veterinärmedicinska Anstalt (SVA)
- Sveriges lantbruksuniversitet, Sveriges lantbruksuniversitet
Sammanfattning
The brain of a stillborn calf, seropositive to Neospora caninum and born to a seropositive cow, was homogenized and cultured on Vero cells, where growth of Neospora-like tachyzoites was detected after 8 weeks. The ultrastructural features of the new isolate (Nc-SweB1) corresponded to those of previously published Neospora isolates. In indirect immunofluorescence tests, antigens on Nc-SweB1 tachyzoites were recognized by antibodies raised to a canine N. caninum isolate (Nc-1) but not by antibodies to Toxoplasma gondii, Sarcocystis cruzi, S. tenella, Eimeria alabamensis, Babesia divergens, or B. motasi. Immunoblot analyses revealed no major antigenic difference between Nc-SweB1 and Nc-1, whereas several differences were seen between Nc-SweB1 and protozoa related to N. caninum. The sequences of 16S-like rRNA and the internal transcribed spacer 1 of Nc-SweB1 revealed complete homology with corresponding sequences of two canine N. caninum isolates. Thus, no dissimilarity between Nc-SweB1 and the canine isolates was found, confirming that Nc-SweB1 is N. caninum and suggesting that Neospora-like organisms isolated from cattle are indeed N. caninum.
Publicerad i
Parasitology Research
1997, volym: 83, nummer: 3, sidor: 214-219
SLU författare
UKÄ forskningsämne
Klinisk vetenskap
Publikationens identifierare
- DOI: https://doi.org/10.1007/s004360050236
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/145157