Ingvarsson, Pär
- Department of Plant Biology, Swedish University of Agricultural Sciences
Abstract
Common problems in biological sample processing for scanning electron microscopy (SEM) include cell collapse and destruction. To overcome the challenges surrounding SEM micrograph preparation, dried rice stems were used to develop a specific set of protocols for processing dried plant samples. Dried stems are rehydrated with a glycerol solution and fixed in formalin-acetic-alcohol to avoid cell wall collapse or organ distortion. The protocols detailed here comprise the first published method for preparing SEM images of dried plant tissue. The protocols offer a cost-effective approach to obtaining high-quality micrographs, facilitating the reconstruction of growth processes and the study of plant cell wall features.
Keywords
anatomical traits; dry sample preparation; image processing; image quantification; Oryza sativa; plant cell wall
Published in
Current protocols
2025, volume: 5, number: 12, article number: e70262
Publisher: WILEY
SLU Authors
UKÄ Subject classification
Botany
Other Biological Topics
Publication identifier
- DOI: https://doi.org/10.1002/cpz1.70262
Permanent link to this page (URI)
https://res.slu.se/id/publ/145620