Ingvarsson, Pär
- Institutionen för växtbiologi, Sveriges lantbruksuniversitet
Sammanfattning
Common problems in biological sample processing for scanning electron microscopy (SEM) include cell collapse and destruction. To overcome the challenges surrounding SEM micrograph preparation, dried rice stems were used to develop a specific set of protocols for processing dried plant samples. Dried stems are rehydrated with a glycerol solution and fixed in formalin-acetic-alcohol to avoid cell wall collapse or organ distortion. The protocols detailed here comprise the first published method for preparing SEM images of dried plant tissue. The protocols offer a cost-effective approach to obtaining high-quality micrographs, facilitating the reconstruction of growth processes and the study of plant cell wall features.
Nyckelord
anatomical traits; dry sample preparation; image processing; image quantification; Oryza sativa; plant cell wall
Publicerad i
Current protocols
2025, volym: 5, nummer: 12, artikelnummer: e70262
Utgivare: WILEY
SLU författare
UKÄ forskningsämne
Botanik
Annan biologi
Publikationens identifierare
- DOI: https://doi.org/10.1002/cpz1.70262
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/145620