Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease

de Jong, Ymke A.; Seren, Rana M.; Ramsak Marceta, Vida; Checa, Antonio; Petursdottir, Dagbjort H.; Badolati, Isabella; Moeckel, Claudia; Ahmed Osman, Omneya; Hell, Eva; Huseby, Douglas L.; Hughes, Diarmaid; Wheelock, Craig E.; Garcia, Sarahi L.; Udekwu, Klas I.; Qazi, Khaleda R.; Sverremark-Ekstrom, Eva

doi:10.1186/s12866-025-04321-9

Forskningsartikel2025Vetenskapligt granskadÖppen tillgång

Impact of early-life human microbiota on the murine host metabolome: insights from a two-generation HMA mouse model and implications for allergic disease

de Jong, Ymke A.; Seren, Rana M.; Ramsak Marceta, Vida; Checa, Antonio; Petursdottir, Dagbjort H.; Badolati, Isabella; Moeckel, Claudia; Ahmed Osman, Omneya; Hell, Eva; Huseby, Douglas L.; Hughes, Diarmaid; Wheelock, Craig E.; Garcia, Sarahi L.; Udekwu, Klas I.; Qazi, Khaleda R.; Sverremark-Ekstrom, Eva

Sammanfattning

IntroductionHuman microbiota-associated (HMA) models are used to allow in vivo studies of the human gut microbiome and its effects on host physiology. In particular, alterations in early life microbiota have been linked to allergy development during childhood. In this study, we investigated how pools of human microbiota collected from infants with different allergy risk, thrive in mice and their offspring, as well as how they influence the host metabolome.MethodWe used a two-generation HMA mouse model in which dams were colonized with human feces from three groups of infants (n = 19, samples collected during the first 8 weeks of life). In two of the groups, all infants had a strong hereditary risk for allergic disease (n = 12), but only 6 of them developed allergy before 2 years of age. In the third group, which was used as a control, none of the infants had allergic heredity or developed allergy (n = 7). Microbiota trajectories were followed from inoculation to mouse offspring, and metabolic profiles were monitored in several intestinal organs as well as in the serum of the murine offspring.ResultsThe human microbiota adapted to the murine host but still presented distinct compositional features, reflecting the original inoculated samples. These microbial differences were mirrored in the mouse offspring metabolome, with group-associated patterns in sphingolipids, acylcarnitines and tryptophan metabolites. Furthermore, the metabolic profiles of the mouse offspring aligned with those observed in fecal water preparations from the corresponding human infant fecal samples.ConclusionOur findings highlight the significant impact of early-life microbiota on the host metabolome and show that our two-generation HMA model is suitable for studying microbiota-metabolome relationships relevant to humans. The differences in microbiota-metabolome correlations between individuals who develop or do not develop allergic disease suggest that an allergic predisposition might be more multifaceted than previously believed.

Nyckelord

Allergy; Infant; Microbiota; Metabolome; Immune profile; Liver; Intestinal tissue; Human microbiota-associated mouse model

Publicerad i

BMC Microbiology
2025, volym: 25, nummer: 1, artikelnummer: 575
Utgivare: BMC

SLU författare

Udekwu, Klas
- University of Idaho

UKÄ forskningsämne

Mikrobiologi

Publikationens identifierare

DOI: https://doi.org/10.1186/s12866-025-04321-9

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/145782