Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation

Sammanfattning

Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants. We describe steps for sterilizing and planting tomato seeds, cotyledon and hypocotyl excision and preculture, Agrobacterium tumefaciens preparation and co-cultivation with explants, and recovering ex-plants and bacterial overgrowth restriction. We then detail procedures for selecting green calli; regenerating explant excision and growth in non-selective media; plant hardening, potting, and growing; and T-DNA insertion confirmation by PCR. For complete details on the use and execution of this protocol, please refer to Mountourak is et al.1-3

Publicerad i

STAR Protocols
2026, volym: 7, nummer: 1, artikelnummer: 104322
Utgivare: CELL PRESS

SLU författare

• Moschou, Panagiotis Nikolaou

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    
  • Foundation for Research and Technology - Hellas (FORTH)
  • Πανεπιστήμιο Κρήτης

UKÄ forskningsämne

Utvecklingsbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1016/j.xpro.2025.104322

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/146000