Ljung, Karin
- Institutionen för skoglig genetik och växtfysiologi, Sveriges lantbruksuniversitet
Sammanfattning
Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In Arabidopsis, the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25S ribosomal RNA (rRNA). Residue m3U2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation.
Publicerad i
Science
2026, volym: 391, nummer: 6786, sidor: 694-699
Utgivare: AMER ASSOC ADVANCEMENT SCIENCE
SLU författare
UKÄ forskningsämne
Genetik och genomik
Cellbiologi
Botanik
Publikationens identifierare
- DOI: https://doi.org/10.1126/science.adx2867
Permanent länk till denna sida (URI)
https://res.slu.se/id/publ/146318