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Sammanfattning

The World Organization for Animal Health (Office International des Epizooties, OIE) includes the diseases caused by foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV), as "Diseases Notifiable to the OIE". Foot-and-mouth disease (FMD) outbreaks have severe economical as well as social effects and cannot be differentiated from the diseases caused by the other two viruses on the basis of clinical symptoms. Efficient laboratory techniques are therefore required for detection and identification of the viruses causing similar vesicular symptoms in swine. A rapid method is described using padlock probes and microarrays to detect simultaneously and differentiate the three viruses in a single reaction, as well as providing serotype information in cases of VSV infection. The padlock probe/microarray assay detected successfully and identified 39 cDNA samples of different origin representing the three viruses. The results were in complete agreement with identities and serotypes determined previously. This novel virus detection method is discussed in terms of usefulness and further development. (C) 2007 Elsevier B.V. All rights reserved.

Nyckelord

foot-and mouth disease; swine vesicular disease; vesicular stomatitis; virus; padlock probes; microarray; rapid detection

Publicerad i

Journal of Virological Methods
2007, volym: 143, nummer: 2, sidor: 200-206
Utgivare: ELSEVIER SCIENCE BV

SLU författare

UKÄ forskningsämne

Veterinärmedicin
Husdjursvetenskap

Publikationens identifierare

  • DOI: https://doi.org/10.1016/j.jviromet.2007.03.004

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/15105